mouse myoblast cell line c2c12  (ATCC)

Journal: Journal of Cellular Physiology

Article Title: PIEZO1 Channels Modulate the Small Extracellular Vesicle Release in C2C12 Cells

doi: 10.1002/jcp.70155

Figure Lengend Snippet: Yoda1‐induced sEV promotes myotube formation. (A) Representative optical field areas of 4‐day differentiating C2C12 cells in control conditions (Ctrl; DM medium), treated with exogenous sEV derived from MB and MT cultures (cMB‐sEV and cMT‐sEV) or with exogenous sEV derived from Yoda1‐treated MB and MT cultures (yMB‐sEV and yMT‐sEV). Fluorescence images (DAPI staining) were overlaid on the corresponding bright‐field images. (B) The number of myotubes in each optical field (OF) was normalized to the average number of myotubes observed in Ctrl condition. For each experimental point, at least 40 randomly selected.

Article Snippet: Myoblasts derived from the C2C12 cell line (RRID: CVCL_0188, ATCC‐CRL‐1772, LCG Standards S.r.l, MI, Italy) were kept in growth media (GM) containing Dulbecco's Modified Eagle Medium‐High Glucose (DMEM, Sigma‐Aldrich, St. Louis, Missouri, USA), 20% Fetal Bovin Serum (FBS; EuroClone, Pero, MI, Italy), 4 mM l ‐Glutamine (EuroClone, Perom MI, Italy), 1% Penicillin‐Streptomycin (EuroClone, Pero, MI, Italy), at 37°C in humidified air containing 5% CO 2 .

Techniques: Control, Derivative Assay, Fluorescence, Staining

Journal: Physiological Reports

Article Title: E‐cigarette exposure impairs skeletal muscle mitochondrial function in male mice

doi: 10.14814/phy2.70797

Figure Lengend Snippet: Tobacco smoke and E‐cig vapor condensates activate the AHR pathway and alter mitochondrial respiration in cultured muscle cells. (a) C2C12 myotubes were treated for 16 h without affecting cell viability. (b–d) mRNA expression of Ahr , Ahrr , and Cyp1a1 in treated myotubes. (e) Schematic overview of mitochondrial assay. (f, g) Mitochondrial respiration assessed using respirometry. Data analyzed using Kruskal‐Wallis test. N = 4–6 biological replicates per group. Error bars represent the standard deviation.

Article Snippet: C2C12 myoblast cell lines were obtained from ATCC (Cat. No. CRL‐1772) and cultured in Dulbecco's Modified Eagle Medium (DMEM, ThermoFisher, Cat. No. 10567022) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, in standard conditions (37°C, 5% CO 2 ).

Techniques: Cell Culture, Expressing, Standard Deviation